Cd68 Mouse Monoclonal Antibody [Clone ID: ED1]

Specifications

Product Data
Clone Name	ED1
Applications	FC, IF, IHC, IP, R, WB
Recommend Dilution	RIA. Western blotting. Immunoprecipitation. Immunofluorescence. Flow Cytometry: Use 10 µl of 1/25-1/100 diluted CD68 antibody to label 106 cells in 100 µl (Membrane permeabilisation is required for best results). Immunohistology on Frozen Sections. Immunohistology on Paraffin Sections: 1/50-1/100. Requires protein digestion pretreatment of Paraffin sections with Pronase or Trypsin.
Reactivity	Bovine, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat spleen cells. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
Specificity	CD68 Antibody (Cat#BM4000) recognises a single chain glycoprotein of 110 kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Cd68 molecule
Database Link	Entrez Gene 287435 Rat UniProt ID Q4FZY1
Background	The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
Synonyms	Gp110, Macrosialin, Macrophage marker
Note	Studies have shown that the antigen recognised by ED1 is the Rat homologue of Human CD68.
Reference Data

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