FITC F(ab)2 Fragment Goat Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF
Recommend Dilution	Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Flow cytometry: 1:2,000 - 1:10,000. Immunoflourescence microscopy: 1:500 - 1:2,500.
Host	Goat
Clonality	Polyclonal
Immunogen	Fluorescein conjugated to Goat IgG
Specificity	This antibody detects Fluorescein F(ab')2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated BSA. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free; 0.01% (w/v) Sodium Azide Label: Texas Red State: Lyophilized Ig fraction Label: Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption emission: 596 nm / 620 nm Molar radio: 3.5 moles Texas RedTM per mole of Goat IgG
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation	Texas Red
Storage Condition	Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Synonyms	Fluorescein Isothiocyanate
Reference Data

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