FITC F(ab)2 Fragment Goat Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF
Recommend Dilution	Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow cytometry: 1:2,000 - 1:10,000. Immunoflourescence: 1:500 - 1:2,500.
Host	Goat
Clonality	Polyclonal
Immunogen	Fluorescein conjugated to Goat IgG
Specificity	This antibody detects Fluorescein F(ab')2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated BSA. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free; 0.01% (w/v) Sodium Azide Label: TRITC State: Lyophilized Ig fraction Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 2.0 moles TRITC per mole of Goat IgG
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
Conjugation	TRITC
Storage Condition	Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Synonyms	Fluorescein Isothiocyanate
Reference Data

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