Ku70/80 (XRCC6/5) Mouse Monoclonal Antibody [Clone ID: KU729]

Specifications

Product Data
Clone Name	KU729
Applications	IF, IHC, IP
Recommend Dilution	Immunofluorescence: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 µg protein lysate. Immunocytochemistry on Acetone or Paraformaldehyde Fixed Sections: 0.25-0.5 μg/ml for 30 minutes at RT. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1.0 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tiss ue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Human cancer K562, HL-60, HEp-2 or HeLa cells.
Reactivity	Human, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Nuclear extract of Human HL-60 cells.
Specificity	Recognizes a dimer of two proteins of 70kDa and ~80kDa, identif ied as two subunits of Ku. This Monoconal Antibody KU729 recognizes a conformational epitope of p70/p80 di mer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase . Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptiona l activation, and apoptosis. Cellular Localization: Nuclear. Negative Species: Mouse, Rat and Chicken.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	70 kDa and 80 kDa
Gene Name	X-ray repair complementing defective repair in Chinese hamster cells 6
Database Link	Entrez Gene 2547 Human UniProt ID P12956
Synonyms	TLAA, G22P2, CTCBF, CTC85
Reference Data

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