ARHGEF4 (143-271) Mouse Monoclonal Antibody [Clone ID: ARHGEF-08]

Specifications

Product Data
Clone Name	ARHGEF-08
Applications	FC, WB
Recommend Dilution	Flow Cytometry: Intracellular staining. Western blot.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant fragment of human ARHGEF4 (amino acids 143-271)
Specificity	The mouse monoclonal antibody ARHGEF-08 recognizes Human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42.
Formulation	PBS, pH~7.4 State: Aff - Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein-A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	Rho guanine nucleotide exchange factor 4
Database Link	Entrez Gene 50649 Human UniProt ID Q9NR80
Background	ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli – stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), Pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.
Synonyms	KIAA1112, ASEF
Reference Data

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