CD175s Mouse Monoclonal Antibody [Clone ID: STN219]

Specifications

Product Data
Clone Name	STN219
Applications	FC, IF, IHC
Recommend Dilution	Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature. Immunofluorescence: 10-20 µg/ml (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow Cytometry: 0.2-1.0 µg/0.1 ml (1/200-1/1,000). Recommended Positive Control: Human gastrointestinal tumor, prostate, ovary ca.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Ovine submaxillary mucin (OSM).
Specificity	The specificity of this antibody is similar to B 72.3 antibody. Cellular Localization: Cytoplasmic, apical membrane, luminal.
Formulation	PBS, pH 7.4 Label: FITC State: Liquid purified Ig fraction. Stabilizer: 1% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Background	Sialosyl Tn antigen also known as CD175s is expressed in the majority of colon, gastric, breast and lung carcinoma. The sialosyl Tn has limited expression to being absent in normal colon, pancreas, stomach, ovary, and prostate. This antigen is detected in several adenocarcinomas. It appears to be useful marker for poorly differentiated colon adenocarcinomas. This antibody stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections.
Synonyms	Sialosyl Tn, Sialosyl-Tn, Sialyl Tn, sTn
Reference Data

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