CD105 (ENG) Mouse Monoclonal Antibody [Clone ID: MEM-229]

Specifications

Product Data
Clone Name	MEM-229
Applications	FC, IF
Recommend Dilution	Flow Cytometry analysis of Human blood cells using 20 µl reagent/100 µl of whole blood or 10e6 cells in a suspension.
Reactivity	Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Vaccinia virus containing the Human CD105 (L-isoform) cDNA
Specificity	This antibody recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.  Negative Species: Canine (Dog), Equine (Horse).
Formulation	Phosphate buffered saline (PBS) containing 15 mM Sodium Azide and 0.2% (w/v) high-grade protease free BSA as a stabilizing agent. Label: PE State: Liquid purified Ig fraction Label: Conjugated with R-Phycoerythrin
Purification	Size-Exclusion Chromatography
Conjugation	PE
Storage Condition	Store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	endoglin
Database Link	Entrez Gene 2022 Human UniProt ID P17813
Background	CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbR-2 as a receptor for TGFb-1 and TGFb-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFb-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
Synonyms	ENG, END, HHT1, ORW, ORW1
Note	The content of a vial (2 ml) is sufficient for 25 tests.
Reference Data

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