CD105 (ENG) Mouse Monoclonal Antibody [Clone ID: MEM-229]

Specifications

Product Data
Clone Name	MEM-229
Applications	FC, IF, IHC, WB
Recommend Dilution	Flow Cytometry: 10 μg/ml. Positive Control: Kg1 Human acute myelogenous leukemia cell line. Western Blotting (Non-Reducing Conditions). Immunocytochemistry. Immunohistochemistry on Frozen Sections: 1/200 (Acetone Fixation).
Reactivity	Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Vaccinia virus containing the Human CD105 (L-isoform) cDNA.
Specificity	The antibody MEM-229 recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Protein-A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	endoglin
Database Link	Entrez Gene 2022 Human UniProt ID P17813
Background	CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFβR-2 as a receptor for TGFβ-1 and TGFβ-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFβ-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
Synonyms	ENG, END, HHT1, ORW, ORW1
Reference Data

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