STAT3 pTyr705 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9E12]

CAT#: AM00148PU-N

STAT3 pTyr705 (incl. pos. control) mouse monoclonal antibody, clone 9E12, Purified

Conjugation: Unconjugated Biotin FITC



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CNY 5,973.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 9E12
Applications ELISA, IF, IHC, IP, WB
Recommend Dilution Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.05 µg/ml.
Immunoprecipitation: 1-10 µg per 106 pervanadate treated HepG2 cells.
Immunocytochemistry: 1-10 µg/ml.
Immunohistochemistry.
Included Positive Control:
HepG2 pervanadate treated Cell lysate (See Protocols for more details).
Reactivity Canine, Human, Mouse
Host Mouse
Clonality Monoclonal
Immunogen Synthetic phosphopeptide conjuagted to KLH.
Epitope: Phosphotyrosine 705 ...A P pY L K...
Specificity The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STAT3 is activated by tyrosine phosphorylation at residue Tyr 705 in cells treated with interleukin 6 or EGF. Activated STAT3 can bind to DNA either as homodimer or as heterodimer with STAT1.
AM00148PU-N specifically recognizes STAT3 phosphorylated at Tyr 705 in human, mouse and dog. The antibody does not crossreact with the non-phosphorylated form of STAT3 nor with unrelated tyrosine-phosphorylated proteins. AM00148PU-N is particularly suitable for Western blot and ELISA applications.
Formulation 1 ml 2 x PBS with 0.09% Sodium Azide, PEG and Sucrose
State: Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Size Exclusion Chromatography
Conjugation Unconjugated
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Predicted Protein Size 92 kDa
Gene Name signal transducer and activator of transcription 3
Background The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT3 is activated by tyrosine phosphorylation at residue Tyr 705 in cells treated with interleukin 6 or EGF. Activated STAT3 can bind to DNA either as homodimer or as heterodimer with STAT1.
Synonyms STAT-3, Acute-phase response factor, APRF
Note Protocol: Positive Control Provided: Cell lysate from HepG2 pervanadate treated.

Format: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15min with pervanadate.

Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Storage: Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.

Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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