STAT3 pTyr705 Mouse Monoclonal Antibody [Clone ID: 9E12]

Specifications

Product Data
Clone Name	9E12
Applications	IF
Recommend Dilution	Immunocytochemistry: 1-10 µg/ml.
Reactivity	Canine, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to KLH Epitope: Phosphotyrosine 705...A P pY L K...
Specificity	This antibody specifically recognizes STAT3 phosphorylated at Tyr705. The antibody does not crossreact with the non-phosphorylated form of STAT3 nor with unrelated tyrosine-phosphorylated proteins.
Formulation	PBS containing 0.09% Sodium Azide, PEG and Sucrose. Label: FITC State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	FITC
Storage Condition	Aliquote and freeze in liquid nitrogen. Avoid repeated freezing and thawing. The antibody is stable at -80°C for one year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Predicted Protein Size	92 kDa
Gene Name	signal transducer and activator of transcription 3
Database Link	Entrez Gene 6774 Human UniProt ID P40763
Background	The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT3 is activated by tyrosine phosphorylation at residue Tyr 705 in cells treated with interleukin 6 or EGF. Activated STAT3 can bind to DNA either as homodimer or as heterodimer with STAT1.
Synonyms	STAT-3, Acute-phase response factor, APRF
Note	For Immunoblotting, Immunoprecipitation or ELISA, clone 9E12 is available as purified or Biotin-labelled antibody (Cat.no. AM00148PU-N or AM00148BT-N).
Reference Data

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