ERK2 (MAPK1) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6G11]

Specifications

Product Data
Clone Name	6G11
Applications	ELISA, IP, WB
Recommend Dilution	ELISA: Use at 0.05 µg/ml. Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Immunoprecipitation: 1-10 µg/ml per 106 pervanadate-treated A431 cells. Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for more details).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to KLH Epitope: C-terminus
Specificity	This antibody specifically recognizes the C-terminus of MAP kinase 2 (ERK2). It does not crossreact with MAP kinase 1 (ERK1).
Formulation	1ml 2 x PBS containing PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore with 1.0 ml H2O (15 min, RT).
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for one month or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	42 kDa
Gene Name	mitogen-activated protein kinase 1
Database Link	Entrez Gene 5594 Human UniProt ID P28482
Background	Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.
Synonyms	Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2, MAPK2, MAPK1
Note	Protocol: Positive Control Provided: Cell lysate from Untreated HepG2 Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human) Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data

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