ERK2 (MAPK1) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6G11]

Specifications

Product Data
Clone Name	6G11
Applications	ELISA, IP, WB
Recommend Dilution	ELISA: Use at 0.05 µg/ml. Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Immunoprecipitation: 1-10 µg/ml per 10e6 pervanadate-treated A431 cells. Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for more details).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to KLH Epitope: C-terminus
Specificity	This antibody specifically recognizes the C-terminus of MAP kinase 2 (ERK2). It does not crossreact with MAP kinase 1 (ERK1).
Formulation	PBS with 0.09% Sodium Azide/PEG and Sucrose. Label: Biotin State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation	Biotin
Storage Condition	Aliquote and freeze in liquid nitrogen Antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	mitogen-activated protein kinase 1
Database Link	Entrez Gene 26413 MouseEntrez Gene 116590 RatEntrez Gene 5594 Human UniProt ID P28482
Background	Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.
Synonyms	Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2, MAPK2, MAPK1
Note	Mol. weight: 42 kDa Protocol: Positive Control Provided. Cell lysate from untreated HepG2 Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human) Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Acute myeloid leukemia, Adherens junction, Alzheimer's disease, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Non-small cell lung cancer, Oocyte meiosis, Pancreatic cancer, Pathways in cancer, Prion diseases, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, TGF-beta signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Type II diabetes mellitus, Vascular smooth muscle contraction, VEGF signaling pathway

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