EGFR Non pTyr1197 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20G3]

Specifications

Product Data
Clone Name	20G3
Applications	ELISA, IF, IP, WB
Recommend Dilution	ELISA (0.05 μg/ml). Western blot. Immunoprecipitation (1 - 10 μg per 10e6 vanadate treated A431 cells). Immunoflourescence (1-10 μg/ml).
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to KLH
Specificity	This antibody specifically recognizes non-activated EGF-receptor (dephosphorylated at Y1197) and is interacting with the 1197 - N A E Y L R V peptide motif. It does not interact with the activated EGF-receptor phosphorylated at Y1197.
Formulation	PBS, 0.09 % Na-azide, PEG and Sucrose State: Purified State: Lyophilized Ig fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent thiophilic adsorption and size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilised product upon arrival at -20 °C. Following reconstitution aliquot and store at 2 - 8 °C for up to three months or freeze in liquid nitrogen at -80 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate, we recommend centrifugation before use.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note	Mol. weight: 180 kDa. Positive control included: Cell lysate from untreated HepG2.
Reference Data

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