EGFR Non pTyr1197 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20G3]

Specifications

Product Data
Clone Name	20G3
Applications	ELISA, IF, IP, WB
Recommend Dilution	ELISA: Use at 0.05 µg/ml Immunoblotting: 1 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Immunocytochemistry: Use at 1-10 µg/ml Immunoprecipitation: Use at 1-10 µg per 10e6 vanadate treated A431 cells. Included Positive Control: Cell lysate from untreated HepG2 (See Protocols for more details).
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to KLH Epitope: Dephospho-Tyr1197
Specificity	This antibody specifically recognizes non-activated EGF-receptor (dephosphorylated at Tyr1197) and is interacting with the 1197 - N A E Y L R V peptide motif. It does not interact with the activated EGF-receptor phosphorylated at Tyr1197.
Formulation	PBS, 0.09% Sodium Azide/PEG and Sucrose. Label: Biotin State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Biotin
Storage Condition	Aliquote and freeze in liquid nitrogen Antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 13649 MouseEntrez Gene 1956 Human UniProt ID P00533
Background	EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note	Mol. weight: 180 kDa. Protocol: Positive Control Provided. Cell lysate from untreated HepG2 Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human) Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Families	Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Secreted Protein, Stem cell relevant signaling - JAK/STAT signaling pathway, Transmembrane
Protein Pathways	Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

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