TrueORF Gold
TrueORF Gold is OriGene’s collection
of premium cDNA clones that have passed the ultimate tests: sequence
verification and protein expression validation. Why settle for clones
with little validation or wait for gene-synthesis? Get a TrueORF Gold
clone and start your experiment immediately.
- Tested individually by Western
- Sequence verified
- Next day shipment
- Transfection ready
- Easily shuttled into over 60 vectors
TrueORF Gold delivers quality and promptness in one tube.
Comparison of cDNA clones from different sources
- Tested individually by Western
Each TrueORF Gold clone has been used to transfect human cell lines for protein expression. In fact, OriGene has produced over 12,000 over-expression lysates from TrueORF-transfected HEK293 cells and subsequently purified over 5,000 human proteins from those cells - Sequence Verified:
Each TrueORF Gold clone is fully sequence with down-loadable chromatogram files posted online. View and analyze the sequences before purchasing and you will never worry about mutations when using TrueORF Gold. Each TrueORFGold clone is shipped out with a 2-GB USB drive storing the supporting data, including the chromatogram files. - Next day shipment
All TrueORF Gold clones are available for next day shipment. Why spend weeks constructing a cDNA clone or have it synthesized? TrueORF Gold accelerates your research and gets you there faster, your experiment, your degree, your publication or your grant application. - Transfection ready:
- No need for subcloning. No need for plasmid preparation. Order a TrueORF Gold clone today, and start your experiment tomorrow.
- TrueORF Gold clones are constructed with all elements (promoter, polyadenylation signal, drug-selection marker, etc) for protein-expression in mammalian cells.
- TrueORF Gold clones are produced using PowerPrep HP ion-exchange columns for high purity and low endotoxicity. Ten ug of purified plasmid DNA are delivered. Larger quantities are available upon request
- Easily shuttled into over 60 vectors
OriGene has prepared over 60 shuttle vectors with matching cloning sites for easy transfer of the insert using PrecisionShuttle, a simple cut-and-paste procedure. Upon transfer, the highly validated inserts are automatically put into a different context for alternative expression options- Fluorescent-protein tagging vectors (27)
- Epitope-tagging vectors (23)
- Selection markers (8)
- Inducible vectors (2)
- Bacterial expression vectors (5)
Sequence-Verification | Expression Validation | Wait Time | Shuttling into 60 vectors | |
---|---|---|---|---|
Yes | Yes | None | Yes | |
Clones constructed in your lab | Y/N | Y/N | Varies | No |
Clones obtained from other vendors | Y/N | No | Varies | No |
Clones obtained by gene-synthesis | Yes | No | Long | No |