pLenti-EF1a-C-tGFP Lentiviral Gene Expression Vector

Specifications

Product Data
C-Tag	tGFP
E. coli Selection	Chloramphenicol (34 ug/mL)
Features	This Lenti vector is a third generation of lentiviral vector. All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. The ORF cloned in this vector can be expressed as a tagged protein with a C-terminal tGFP tag. The expression of the cloned ORF is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment. A brief lentiviral packaging protocol is provided. E. Coli selection: Chloramphenicol(34ug/ml) EF51 Primer can be used to sequence the target sequence after cloning. AC_GFP_R Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites

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