pCas-Guide-Nickase (D10A)

CAT＃: GE100019

pCas-Guide-Nickase vector for gRNA cloning (with Cas9 D10A expression) (10 µg)

Datasheet
SDS
Vector Sequence

CN¥ 4,660.00

货期*
现货
规格
    • 10 ug
Cited in 1 publication.

Product images

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Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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TP790151
Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Specifications

Product Data
Function Cas9 Nickase
Features

All-in-one vector

  • Expresses human codon-optimized Cas9 nickase D10A mutant, C-terminal Myc-DDK tagged
  • BamH I and BsmB I can be used for genomic target sequence cloning
  • Expresses gRNA containing the inserted target sequence and the gRNA scaffold
  • CF3 primer can be used to sequence the target sequence after cloning
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this Vectors has been cited in the following citations:
CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing ,null, Frontiers in Plant Science ,PubMed ID 27917188

Documents

Product Manuals
FAQs
SDS
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