pCas-Guide-EF1a-GFP

CAT＃: GE100018

pCas-Guide-EF1a-GFP vector (with Cas9 and GFP expression) for genomic target sequence cloning (10 µg)

Datasheet
SDS
Vector Sequence

CNY 4,560.00

货期*
现货
规格
    • 10 ug
Cited in 27 publications.

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【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TP790148
Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
    • 500 pmol

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TP790151
Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
    • 500 pmol

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添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售

Specifications

Product Data
Function All-in-One CRISPR/Cas9 vectors
Features
  • All-in-one vector
    • Expresses human codon-optimized Cas9, C-terminal Myc-DDK tagged
    • BamH I and BsmB I can be used for genomic target sequence cloning
    • Expresses gRNA containing the inserted target sequence and the gRNA scaffold
    • GFP as a reporter for tracking or sorting transfected cells
    • CF3 primer can be used to sequence the target sequence after cloning
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (27)

The use of this Vectors has been cited in the following citations:
Raman spectroscopy reveals phenotype switches in breast cancer metastasis ,null, Theranostics ,PubMed ID 35910801
Regulation of closely juxtaposed protooncogene c-fms and HMGXB3 gene expression by mRNA 3\'end polymorphism in breast cancer cells ,Woo, HH;Chambers, SK;, RNA (New York, N.Y.) 2021 ,PubMed ID 34155128 [pCas-Guide-EF1a-GFP]
Molecular Mechanisms for Krüppel-Like Factor 13 Actions in Hippocampal Neurons. ,null, Molecular neurobiology ,PubMed ID 32578009
Identification of the Bok Interactome Using Proximity Labeling ,null, Frontiers in Cell and Developmental Biology ,PubMed ID 34136494
A G316A Polymorphism in the Ornithine Decarboxylase Gene Promoter Modulates MYCN-Driven Childhood Neuroblastoma ,Gamble, L;Purgato, S;Henderson, M;Di Giacomo, S;Russell, A;Pigini, P;Murray, J;Valli, E;Milazzo, G;Giorgi, F;Cowley, M;Ashton, L;Bhalshankar, J;Schleiermacher, G;Rihani, A;Van Maerken, T;Vandesompele, J;Speleman, F;Versteeg, R;Koster, J;Eggert, A;Noguera, R;Stallings, R;Tonini, G;Fong, K;Vaksman, Z;Diskin, S;Maris, J;London, W;Marshall, G;Ziegler, D;Hogarty, M;Perini, G;Norris, M;Haber, M;, Cancers ,PubMed ID 33918978 [pCas-Guide-EF1a-GFP]
Impaired flickering of the permeability transition pore causes SPG7 spastic paraplegia ,Sambri, I;Massa, F;Gullo, F;Meneghini, S;Cassina, L;Carraro, M;Dina, G;Quattrini, A;Patanella, L;Carissimo, A;Iuliano, A;Santorelli, F;Codazzi, F;Grohovaz, F;Bernardi, P;Becchetti, A;Casari, G;, EBioMedicine ,PubMed ID 33045469 [pCas-Guide-EF1a-GFP]
MicroRNA-dependent inhibition of PFN2 orchestrates ERK activation and pluripotent state transitions by regulating endocytosis ,Sangokoya, C;Blelloch, R;, bioRxiv [pCas-Guide-EF1a-GFP]
Modulation of biliary cancer chemo-resistance through microRNA-mediated rewiring of the expansion of CD133+ cells ,Carotenuto, P;Hedayat, S;Fassan, M;Cardinale, V;Lampis, A;Guzzardo, V;Vicentini, C;Scarpa, A;Cascione, L;Costantini, D;Carpino, G;Alvaro, D;Ghidini, M;Trevisani, F;Te Poele, R;Salati, M;Ventura, S;Vlachogiannis, G;Hahne, JC;Boulter, L;Forbes, SJ;Guest, R;Cillo, U;Said-Huntingford, I;Begum, R;Smyth, E;Michalarea, V;Cunningham, D;Rimassa, L;Santoro, A;Roncalli, M;Kirnkin, V;Clarke, P;Workman, P;Valeri, N;Braconi, C;, Hepatology ,PubMed ID 31879968 [pCas-Guide-EF1a-GFP]
Generation of a GLA knock-out human-induced pluripotent stem cell line, KSBCi002-A-1, using CRISPR/Cas9 ,Kim, YK;Yu, JH;Min, SH;Park, SW;, Stem Cell Res ,PubMed ID 31841972 [pCas-Guide-EF1a-GFP]
Developing oncolytic Herpes simplex virus type 1 through UL39 knockout by CRISPR-Cas9 ,null, Iranian Journal of Basic Medical Sciences ,PubMed ID 32774817
Abi1 loss drives prostate tumorigenesis through activation of EMT and non-canonical WNT signaling ,Nath, D;Li, X;Mondragon, C;Post, D;Chen, M;White, JR;Hryniewicz-Jankowska, A;Caza, T;Kuznetsov, VA;Hehnly, H;Jamaspishvili, T;Berman, DM;Zhang, F;Kung, SHY;Fazli, L;Gleave, ME;Bratslavsky, G;Pandolfi, PP;Kotula, L;, Cell Commun. Signal ,PubMed ID 31530281 [pCas-Guide-EF1a-GFP]
Inclusion of the viral pentamer complex in a vaccine design greatly improves protection against congenital cytomegalovirus in the guinea pig model ,Choi, KY;El-Hamdi, NS;McGregor, A;, J. Virol. ,PubMed ID 31484753 [pCas-Guide-EF1a-GFP]
A requirement for STAG2 in replication fork progression creates a targetable synthetic lethality in cohesin-mutant cancers ,null, Nature Communications ,PubMed ID 30975996
Pathogenic variants in the AFG3L2 proteolytic domain cause SCA28 through haploinsufficiency and proteostatic stress-driven OMA1 activation ,null, Journal of Medical Genetics ,PubMed ID 30910913
Genetic engineering a large animal model of human hypophosphatasia in sheep ,Williams, DK;Pinzón, C;Huggins, S;Pryor, JH;Falck, A;Herman, F;Oldeschulte, J;Chavez, MB;Foster, BL;White, SH;Westhusin, ME;Suva, LJ;Long, CR;Gaddy, D;, Sci Rep ,PubMed ID 30446691 [pCas-Guide-EF1a-GFP]
Tumor-derived granulocyte colony-stimulating factor diminishes efficacy of breast tumor cell vaccines ,Ravindranathan, S;Nguyen, KG;Kurtz, SL;Frazier, HN;Smith, SG;Koppolu, BP;Rajaram, N;Zaharoff, DA;, Breast Cancer Res. ,PubMed ID 30348199 [pCas-Guide-EF1a-GFP]
Nuclear receptor/Wnt beta-catenin interactions are regulated via differential CBP/p300 coactivator usage ,Ono, M;Lai, KKY;Wu, K;Nguyen, C;Lin, DP;Murali, R;Kahn, M;, PLoS ONE ,PubMed ID 30020971 [pCas-Guide-EF1a-GFP]
Interplay between ER Ca2+ Binding Proteins, STIM1 and STIM2, Is Required for Store-Operated Ca2+ Entry ,Nelson, HA;Leech, CA;Kopp, RF;Roe, MW;, Int J Mol Sci ,PubMed ID 29783744 [pCas-Guide-EF1a-GFP]
APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway ,Saito-Diaz, K;Benchabane, H;Tiwari, A;Tian, A;Li, B;Thompson, JJ;Hyde, AS;Sawyer, LM;Jodoin, JN;Santos, E;Lee, LA;Coffey, RJ;Beauchamp, RD;Williams, CS;Kenworthy, AK;Robbins, DJ;Ahmed, Y;Lee, E;, Dev. Cell ,PubMed ID 29533772 [pCas-Guide-EF1a-GFP]
A novel mechanism of lncRNA and miRNA interaction: CCAT2 regulates miR-145 expression by suppressing its maturation process in colon cancer cells ,Yu, Y;Nangia-Makker, P;Farhana, L;Majumdar, APN;, Mol. Cancer ,PubMed ID 28964256 [pCas-Guide-EF1a-GFP]
Potent In Vivo NK Cell-mediated Elimination of HIV-1-Infected Cells Mobilized by a gp120-bispecific and Hexavalent Broadly Neutralizing Fusion Protein ,Bardhi, A;Wu, Y;Chen, W;Zhu, Z;Zheng, JH;Wong, H;Jeng, E;Jones, J;Ochsenbauer, C;Kappes, JC;Dimitrov, DS;Ying, T;Goldstein, H;, J. Virol. ,PubMed ID 28794022 [pCas-Guide-EF1a-GFP]
CTCF facilitates DNA double-strand break repair by enhancing homologous recombination repair ,Hilmi, K;Jangal, M;Marques, M;Zhao, T;Saad, A;Zhang, C;Luo, VM;Syme, A;Rejon, C;Yu, Z;Krum, A;Fabian, MR;Richard, S;Alaoui-Jamali, M;Orthwein, A;McCaffrey, L;Witcher, M;, Sci Adv ,PubMed ID 28560323 [pCas-Guide-EF1a-GFP]
The Krüppel-like factor 9 cistrome in mouse hippocampal neurons reveals predominant transcriptional repression via proximal promoter binding ,Knoedler, JR;Subramani, A;Denver, RJ;, BMC Genomics ,PubMed ID 28407733 [CRISPR]
Eradication of Tumors through Simultaneous Ablation of CD276/B7-H3-Positive Tumor Cells and Tumor Vasculature ,Seaman, S;Zhu, Z;Saha, S;Zhang, XM;Yang, MY;Hilton, MB;Morris, K;Szot, C;Morris, H;Swing, DA;Tessarollo, L;Smith, SW;Degrado, S;Borkin, D;Jain, N;Scheiermann, J;Feng, Y;Wang, Y;Li, J;Welsch, D;DeCrescenzo, G;Chaudhary, A;Zudaire, E;Klarmann, KD;Keller, JR;Dimitrov, DS;St Croix, B;, Cancer Cell ,PubMed ID 28399408 [pCas-Guide-GFP]
Gene signature driving invasive mucinous adenocarcinoma of the lung ,Guo, M;Tomoshige, K;Meister, M;Muley, T;Fukazawa, T;Tsuchiya, T;Karns, R;Warth, A;Fink-Baldauf, IM;Nagayasu, T;Naomoto, Y;Xu, Y;Mall, MA;Maeda, Y;, EMBO Mol Med ,PubMed ID 28255028 [pCas-Guide-EF1a-GFP]
Hypertension Causing Mutations in Cullin3 Impair RhoA Ubiquitination and Augment Association with Substrate Adaptors ,Ibeawuchi, SC;Agbor, LN;Quelle, FW;Sigmund, CD;, J. Biol. Chem. ,PubMed ID 26100637 [CRISPR Vector]
Regulation of vascular function: roles for Cullin-3 and RhoBTB1 In Ppar?-mediated blood pressure control ,Ibeawuchi, SRC;, Thesis [CRISPR Vector]

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