pCas-Guide

CAT＃: GE100002

pCAS-Guide vector (with Cas9 expression) for genomic target sequence cloning (10 µg), GE100008 included.

Datasheet
SDS
Vector Sequence
GE100002 is the updated version of GE100001.

CNY 4,560.00

货期*
4周
规格
    • 10 ug
Cited in 35 publications.

Product images

经常一起买 (4)
TA811179
CAS9 mouse monoclonal antibody,clone OTI5E5
    • 100 ul

CNY 1,999.00
CNY 2,700.00

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【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TP790148
Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
    • 500 pmol

CNY 1,020.00

添加到购物车
TP790151
Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
    • 500 pmol

CNY 1,020.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售

Specifications

Product Data
Function All-in-One CRISPR/Cas9 vectors
Features
  • All-in-one vector
    1. Expresses human codon-optimized Cas9
    2. BamH I and BsmB I can be used for genomic target sequence cloning
    3. Expresses gRNA containing the inserted target sequence and the gRNA scaffold
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (35)

The use of this Vectors has been cited in the following citations:
Caprin-1 binding to the critical stress granule protein G3BP1 is influenced by pH ,null, Open Biology ,PubMed ID 37161291
Dual inhibition of IDO1/TDO2 enhances anti-tumor immunity in platinum-resistant non-small cell lung cancer. ,null, Cancer & metabolism ,PubMed ID 37226257
Transcriptomic and Proteomic Analysis of CRISPR/Cas9-Mediated ARC-Knockout HEK293 Cells ,null, International Journal of Molecular Sciences ,PubMed ID 35562887
Selective Cleavage at CCA Ends and Anticodon Loops of tRNAs by Stress-Induced RNases ,null, Frontiers in Molecular Biosciences ,PubMed ID 35300117
Early rRNA processing is a stress-dependent regulatory event whose inhibition maintains nucleolar integrity ,null, Nucleic Acids Research ,PubMed ID 34928368
Accumulation of APP-CTF induces mitophagy dysfunction in the iNSCs model of Alzheimer’s disease ,null, Cell Death Discovery ,PubMed ID 35013145
PDIA3 inhibits mitochondrial respiratory function in brain endothelial cells and C. elegans through STAT3 signaling and decreases survival after OGD ,null, Cell Communication and Signaling : CCS ,PubMed ID 34922569
MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells ,null, International Journal of Molecular Sciences ,PubMed ID 34829999
Development of a versatile HPLC-based method to evaluate the activation status of small GTPases ,null, The Journal of Biological Chemistry ,PubMed ID 34801548
Core circadian clock transcription factor BMAL1 regulates mammary epithelial cell growth, differentiation, and milk component synthesis ,null, PLoS ONE ,PubMed ID 34415905
Generation of an NANS homozygous knockout human induced pluripotent stem cell line by the insertion of GFP-P2A-Puro via CRISPR/Cas9 editing. ,null, Stem cell research ,PubMed ID 33130469
Efficient viral delivery of Cas9 into human safe harbor ,Hayashi, H;Kubo, Y;Izumida, M;Matsuyama, T;, Scientific reports ,PubMed ID 33293588 [pCAS-Guide]
Stress Granule Assembly Can Facilitate but Is Not Required for TDP-43 Cytoplasmic Aggregation ,Fernandes, N;Nero, L;Lyons, SM;Ivanov, P;Mittelmeier, TM;Bolger, TA;Buchan, JR;, Biomolecules ,PubMed ID 32992901 [pCas-Guide]
Selenoprotein N is an endoplasmic reticulum calcium sensor that links luminal calcium levels to a redox activity ,null, Proceedings of the National Academy of Sciences of the United States of America ,PubMed ID 32817544
Human Leukocyte Antigen Class I Pseudo-Homozygous Mesenchymal Stem Cells Derived from Human Induced Pluripotent Stem Cells ,Kwon, D;Ahn, HJ;Han, MJ;Ji, M;Ahn, J;Seo, KW;Kang, KS;, Stem Cell Rev Rep ,PubMed ID 32712868 [pCAS-Guide]
Histone deacetylase inhibitors induce medulloblastoma cell death independent of HDACs recruited in REST repression complexes. Histone deacetylase inhibitors induce medulloblastoma cell death independent of HDACs recruited in REST repression complexes ,null, Molecular Genetics & Genomic Medicine ,PubMed ID 32720471
USP42 enhances homologous recombination repair by promoting R-loop resolution with a DNA-RNA helicase DHX9 ,Matsui, M;Sakasai, R;Abe, M;Kimura, Y;Kajita, S;Torii, W;Katsuki, Y;Ishiai, M;Iwabuchi, K;Takata, M;Nishi, R;, Oncogenesis ,PubMed ID 32541651 [pCAS-Guide]
In Vitro Impact of FSH Glycosylation Variants on FSH Receptor-stimulated Signal Transduction and Functional Selectivity ,null, Journal of the Endocrine Society ,PubMed ID 32342021
Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization ,Sanders, DW;Kedersha, N;Lee, DSW;Strom, AR;Drake, V;Riback, JA;Bracha, D;Eeftens, JM;Iwanicki, A;Wang, A;Wei, MT;Whitney, G;Lyons, SM;Anderson, P;Jacobs, WM;Ivanov, P;Brangwynne, CP;, Cell ,PubMed ID 32302570 [pCAS-Guide]
PTPRS drives adaptive resistance to MEK/ERK inhibitors through SRC ,null, Oncotarget ,PubMed ID 31827720
Multiple ribonuclease A family members cleave transfer RNAs in response to stress ,Akiyama, Y;Lyons, SM;Fay, MM;Abe, T;Anderson, P;, bioRxiv [pCAS-Guide]
Ser-Phosphorylation of PCSK9 (Proprotein Convertase Subtilisin-Kexin 9) by Fam20C (Family With Sequence Similarity 20, Member C) Kinase Enhances Its Ability to Degrade the LDLR (Low-Density Lipoprotein Receptor) ,Ben Djoudi Ouadda, A;Gauthier, MS;Susan-Resiga, D;Girard, E;Essalmani, R;Black, M;Marcinkiewicz, J;Forget, D;Hamelin, J;Evagelidis, A;Ly, K;Day, R;Galarneau, L;Corbin, F;Coulombe, B;Çaku, A;Tagliabracci, VS;Seidah, NG;, Arterioscler. Thromb. Vasc. Biol. ,PubMed ID 31553664 [pCas-Guide]
A novel nuclear speckle factor, USP42, promotes homologous recombination repair by resolving DNA double-strand break induced R-loop ,Matsui, M;Sakasai, R;Abe, M;Kimura, Y;Kajita, S;Torii, W;, bioRxiv [pCas-Guide]
PTPRS Regulates Colorectal Cancer RAS Pathway Activity by Inactivating Erk and Preventing Its Nuclear Translocation ,null, Scientific Reports ,PubMed ID 29915291
Metformin Targets Mitochondrial Glycerophosphate Dehydrogenase to Control Rate of Oxidative Phosphorylation and Growth of Thyroid Cancer in vitro and in vivo ,null, Clinical cancer research : an official journal of the American Association for Cancer Research ,PubMed ID 29691295
Linear mitochondrial DNA is rapidly degraded by components of the replication machinery ,Peeva, V;Blei, D;Trombly, G;Corsi, S;Szukszto, MJ;Rebelo-Guiomar, P;Gammage, PA;Kudin, AP;Becker, C;Altmüller, J;Minczuk, M;Zsurka, G;Kunz, WS;, Nat Commun ,PubMed ID 29712893 [pCAS-Guide]
APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway ,null, Developmental cell ,PubMed ID 29533772
Generation of PD-L1-Deficient Cell Line by AAVMediated CRISPR Knockout for Ribosome Display Selection ,Ivanova, AE;Kravchenko, DS;, ProQuest [pCAS-Guide]
Potent In Vivo NK Cell-Mediated Elimination of HIV-1-Infected Cells Mobilized by a gp120-Bispecific and Hexavalent Broadly Neutralizing Fusion Protein ,null, Journal of Virology ,PubMed ID 28794022
Helicobacter pylori modulates host cell responses by CagT4SS-dependent translocation of an intermediate metabolite of LPS inner core heptose biosynthesis ,null, PLoS Pathogens ,PubMed ID 28715499
Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis ,Jiang, X;Jiang, X;Feng, Y;Xu, R;Wang, Q;Deng, H;, PLoS ONE ,PubMed ID 27959964 [pCas-Guide]
Molecular changes in appearance of a cancer cell among normal HEK293T cells ,Chung, J;Aburaya, S;Aoki, W;Ueda, M;, J. Biosci. Bioeng. ,PubMed ID 27777049 [pCas-Guide]
YB-1 regulates tiRNA-induced Stress Granule formation but not translational repression ,null, Nucleic Acids Research ,PubMed ID 27174937
YB-1 regulates tiRNA-induced Stress Granule formation but not translational repression ,Shawn M. Lyons, Chris Achorn, Nancy L. Kedersha, Paul J. Anderson, and Pavel Ivanov, Nucleic Acids Res., May 2016; 10.1093/nar/gkw418. [CRISPR vector]
G3BP–Caprin1–USP10 complexes mediate stress granule condensation and associate with 40S subunits ,null, The Journal of Cell Biology ,PubMed ID 27022092

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