BRUNOL4 (CELF4) Human siRNA Oligo Duplex (Locus ID 56853)

Specifications

Product Data
Purity	HPLC purified
Quality Control	Tested by ESI-MS
Sequences	Available with shipment
Stability	One year from date of shipment when stored at -20°C.
# of transfections	Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note	Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq	NM_001025087, NM_001025088, NM_001025089, NM_020180, NM_001330603, NM_001353695, NM_001353696, NM_001353697, NM_001353698, NM_001353699, NM_001353700, NM_001353701, NM_001353702, NM_001353703, NM_001353705, NM_001353706, NM_001353707, NM_001353708, NM_001353709, NM_001353710, NM_001353711, NM_001353712, NM_001353713, NM_001353714, NM_001353715, NM_001353716, NM_001353717, NM_001353718, NM_001353719, NM_001353720, NM_001353721, NM_001353722, NM_001353723, NM_001353724, NM_001353725, NM_001353726, NM_001353727, NM_001353728, NM_001353729, NM_001353730, NM_001353731, NM_001353732, NM_001353733, NM_001353734, NM_001353735, NM_001353736, NM_001353737, NM_001353738, NM_001353739, NM_001353740, NM_001353741, NM_001353742, NM_001353743, NM_001353744, NM_001353745, NM_001353746, NM_001353747, NM_001353748, NM_001353749, NM_001353750, NM_001353751, NM_001353752, NM_001353753, NM_001353754, NM_001353755, NM_001353756, NM_001353757, NM_001353758, NM_001353759, NM_001353760, NM_001353761, NR_148518, NR_148519, NR_148520, NR_148521, NR_148522, NR_148523, NR_148524, NR_148525, NR_148526, NR_148527, NR_148528
Synonyms	BRUNOL4; CELF-4
Components	CELF4 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 56853) Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Summary	Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Performance Guranteed	OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.