Dicer (DICER1) Human siRNA Oligo Duplex (Locus ID 23405)
CAT#: SR323577
DICER1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
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SR323577 is the updated version of SR308251.
CNY 4,090.00
货期*
4周
规格
Cited in 1 publication. |
Product images
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经常一起买 (3)
DICER1 mouse monoclonal antibody, clone OTI1G8 (formerly 1G8)
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Purity | HPLC purified |
Quality Control | Tested by ESI-MS |
Sequences | Available with shipment |
Stability | One year from date of shipment when stored at -20°C. |
# of transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Note | Single siRNA duplex (10nmol) can be ordered. |
Reference Data | |
RefSeq | NM_001195573, NM_001271282, NM_001291628, NM_030621, NM_177438 |
Synonyms | DCR1; Dicer; Dicer1e; GLOW; HERNA; K12H4.8-LIKE; MNG1; RMSE2 |
Components | DICER1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 23405)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml |
Summary | This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010] |
Performance Guranteed | OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required). |
Citations (1)
The use of this RNAi has been cited in the following citations: |
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Identification of PTBP1 responsible for caspase dependent YRNA cleavage
,Ogata, J;Sugiura, Y;Kanai, A;Tanaka, M;Matsui, H;Ohtsuka, M;Inaba, T;Otsuka, M;Kotani, A;,
bioRxiv
[DICER1]
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