Rabex5 (RABGEF1) Human siRNA Oligo Duplex (Locus ID 27342)

Specifications

Product Data
Purity	HPLC purified
Quality Control	Tested by ESI-MS
Sequences	Available with shipment
Stability	One year from date of shipment when stored at -20°C.
# of transfections	Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note	Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq	NM_001287060, NM_001287061, NM_001287062, NM_014504, NR_104676, NM_001367755, NM_001367724, NM_001367726, NM_001367727, NM_001367729, NM_001367730, NM_001367733, NM_001367734, NM_001367736, NM_001367738, NM_001367739, NM_001367740, NM_001367741, NM_001367742, NM_001367745, NM_001367746, NM_001367748, NM_001367749, NM_001367751, NM_001367752, NM_001367753, NM_001367756, NR_160287, NM_001367722, NM_001367723, NM_001367725, NM_001367728, NM_001367731, NM_001367732, NM_001367735, NM_001367737, NM_001367743, NM_001367744, NM_001367747, NM_001367750, NM_001367754
Synonyms	rabex-5; RABEX5; RAP1
Components	RABGEF1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 27342) Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Summary	RABGEF1 forms a complex with rabaptin-5 (RABPT5; MIM 603616) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5 (RAB5A; MIM 179512) (Horiuchi et al., 1997 [PubMed 9323142]).[supplied by OMIM, Mar 2010]
Performance Guranteed	OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.