Ankyrin brain (ANK2) Human siRNA Oligo Duplex (Locus ID 287)

Specifications

Product Data
Purity	HPLC purified
Quality Control	Tested by ESI-MS
Sequences	Available with shipment
Stability	One year from date of shipment when stored at -20°C.
# of transfections	Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note	Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq	NM_001127493, NM_001148, NM_020977, NM_001354225, NM_001354228, NM_001354230, NM_001354231, NM_001354232, NM_001354235, NM_001354236, NM_001354237, NM_001354239, NM_001354240, NM_001354241, NM_001354242, NM_001354243, NM_001354244, NM_001354245, NM_001354246, NM_001354249, NM_001354252, NM_001354253, NM_001354254, NM_001354255, NM_001354256, NM_001354257, NM_001354258, NM_001354260, NM_001354261, NM_001354262, NM_001354264, NM_001354265, NM_001354266, NM_001354267, NM_001354268, NM_001354269, NM_001354270, NM_001354271, NM_001354272, NM_001354273, NM_001354274, NM_001354275, NM_001354276, NM_001354277, NM_001354278, NM_001354279, NM_001354280, NM_001354281, NM_001354282
Synonyms	ANK-2; brank-2; CFAP87; FAP87; LQT4
Components	ANK2 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 287) Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Summary	This gene encodes a member of the ankyrin family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton. Ankyrins play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. The protein encoded by this gene is required for targeting and stability of Na/Ca exchanger 1 in cardiomyocytes. Mutations in this gene cause long QT syndrome 4 and cardiac arrhythmia syndrome. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Dec 2011]
Performance Guranteed	OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.