Sytl2 Mouse shRNA Plasmid (Locus ID 83671)

Specifications

Product Data
Product Name	Sytl2 Mouse shRNA Plasmid (Locus ID 83671)
Locus ID	83671
UniProt ID	Q99N50
Synonyms	AI266830; mKIAA1597; Slp2
Vector	pRS
Format	Retroviral plasmids
Kit Components	Sytl2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 83671). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001040085, NM_001040086, NM_001040087, NM_001040088, NM_001289583, NM_001289584, NM_001289585, NM_001289586, NM_031394, NR_110348, NM_001040085.1, NM_001040085.2, NM_001040088.1, NM_001040088.2, NM_001040086.1, NM_031394.1, NM_031394.2, NM_031394.3, NM_001040087.1, NM_001040087.2, NM_001289585.1, NM_001289586.1, NM_001289584.1, NM_001289583.1, BC138714, BC145398, BC052915, BC060665, BC064769, NM_001368878
Summary	Isoform 11 acts as a RAB27A effector protein and plays a role in cytotoxic granule exocytosis in lymphocytes. Required for cytotoxic granule docking at the immunologic synapse. Isoform 1 may play a role in melanosome transport and vesicle trafficking. It controls melanosome distribution in the cell periphery and regulates melanocyte morphology. Isoform 1 acts as a positive mediator of mucus secretion by the surface mucus cells of the stomach. Mediates basal mucus secretion by gastric surface cells by promoting the proper granule biognesis and docking of mucus granules with the apical plasma membrane.[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.