Spry4 Mouse shRNA Plasmid (Locus ID 24066)
CAT#: TR509780
Spry4 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
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CNY 4,790.00
货期*
现货
规格
Cited in 2 publications. |
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经常一起买 (1)
Specifications
Product Data | |
Product Name | Spry4 Mouse shRNA Plasmid (Locus ID 24066) |
Locus ID | 24066 |
UniProt ID | Q9WTP2 |
Synonyms | A030006O18Rik; sprouty4 |
Vector | pRS |
Format | Retroviral plasmids |
Kit Components | Spry4 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 24066). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free. |
RefSeq | BC050944, BC057005, NM_011898, NM_011898.1, NM_011898.2, NM_011898.3 |
Summary | Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras (By similarity). Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 (By similarity).[UniProtKB/Swiss-Prot Function] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (2)
The use of this RNAi has been cited in the following citations: |
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Novel Function of Sprouty4 as a Regulator of Stemness and Differentiation of Embryonic Stem Cells
,null,
Development & Reproduction
,PubMed ID 27660833
[Spry4]
|
Suppression of Sproutys Has a Therapeutic Effect for a Mouse Model of Ischemia by Enhancing Angiogenesis
,Koji Taniguchi, Ken-ichiro Sasaki, Kousuke Watari, Hideo Yasukawa, Tsutomu Imaizumi, Toranoshin Ayada, Fuyuki Okamoto, Takuma Ishizaki,Reiko Kato,Ri-ichiro Kohno,Hiroshi Kimura,Yasufumi Sato,Mayumi Ono,Yoshikazu Yonemitsu,and Akihiko Yoshimura,
PLoS ONE. 2009; 4(5): e5467
[Spry4]
|
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