LXR alpha (NR1H3) Human shRNA Plasmid Kit (Locus ID 10062)

Specifications

Product Data
Product Name	LXR alpha (NR1H3) Human shRNA Plasmid Kit (Locus ID 10062)
Locus ID	10062
UniProt ID	Q13133
Synonyms	LXR-a; LXRA; RLD-1
Vector	pRS
Format	Retroviral plasmids
Kit Components	NR1H3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 10062). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001130101, NM_001130102, NM_001251934, NM_001251935, NM_005693, NM_005693.1, NM_005693.3, NM_001130101.1, NM_001130101.2, NM_001130102.1, NM_001130102.2, NM_001251934.1, NM_001251935.1, BC008819, BC041172, BM835230, NM_001363595, NM_005693.4, NM_001130102.3, NM_001130101.3
Summary	The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.