CERKL Human shRNA Plasmid Kit (Locus ID 375298)

Specifications

Product Data
Product Name	CERKL Human shRNA Plasmid Kit (Locus ID 375298)
Locus ID	375298
UniProt ID	Q49MI3
Synonyms	RP26
Vector	pRS
Format	Retroviral plasmids
Kit Components	CERKL - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 375298). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001030311, NM_001030312, NM_001030313, NM_001030314, NM_001030315, NM_001160277, NM_201548, NR_027689, NR_027690, NM_001030311.1, NM_001030311.2, NM_001030312.2, NM_001030313.1, NM_001030313.2, NM_201548.1, NM_201548.2, NM_201548.3, NM_201548.4, NM_001160277.1, NM_001030314.1, NM_001030315.1, BC137498, BC137499, BM969366
Summary	This gene was initially identified as a locus (RP26) associated with an autosomal recessive form of retinitis pigmentosa (arRP) disease. This gene encodes a protein with ceramide kinase-like domains, however, the protein does not phosphorylate ceramide and its target substrate is currently unknown. This protein may be a negative regulator of apoptosis in photoreceptor cells. Mutations in this gene cause a form of retinitis pigmentosa characterized by autosomal recessive cone and rod dystrophy (arCRD). Alternative splicing of this gene results in multiple transcript variants encoding different isoforms and non-coding transcripts.[provided by RefSeq, May 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.