MBD1 Human shRNA Plasmid Kit (Locus ID 4152)

Specifications

Product Data
Product Name	MBD1 Human shRNA Plasmid Kit (Locus ID 4152)
Locus ID	4152
UniProt ID	Q9UIS9
Synonyms	CXXC3; PCM1; RFT
Vector	pRS
Format	Retroviral plasmids
Kit Components	MBD1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 4152). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001204136, NM_001204137, NM_001204138, NM_001204139, NM_001204140, NM_001204141, NM_001204142, NM_001204143, NM_001204151, NM_002384, NM_015844, NM_015845, NM_015846, NM_015847, NM_001323942, NM_001323947, NM_001323949, NM_001323950, NM_001323951, NM_001323952, NM_001323953, NM_001323954, NM_015844.1, NM_015844.2, NM_015846.1, NM_015846.2, NM_015846.3, NM_015845.1, NM_015845.2, NM_015845.3, NM_015847.1, NM_015847.2, NM_015847.3, NM_002384.1, NM_002384.2, NM_001204143.1, NM_001204151.1, NM_001204151.2, NM_001204142.1, NM_001204141.1, NM_001204140.1, NM_001204139.1, NM_001204138.1, NM_001204137.1, NM_001204136.1, BC033242, BC033242.1, BC047353, NM_001204140.2, NM_001204137.2, NM_001204151.3, NM_001204142.2, NM_001204143.2, NM_001204138.2
Summary	The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.