NBR1 Human shRNA Plasmid Kit (Locus ID 4077)

Specifications

Product Data
Product Name	NBR1 Human shRNA Plasmid Kit (Locus ID 4077)
Locus ID	4077
UniProt ID	Q14596
Synonyms	1A1-3B; IAI3B; M17S2; MIG19
Vector	pRS
Format	Retroviral plasmids
Kit Components	NBR1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 4077). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	BC009808, NM_001291571, NM_001291572, NM_005899, NM_031858, NM_031862, NM_031862.1, NM_031862.2, NM_031862.3, NM_005899.1, NM_005899.2, NM_005899.3, NM_005899.4, NM_031858.1, NM_031858.2, NM_001291572.1, NM_001291571.1, BC009808.2, BC012591, BC012847, BM509256, NM_001291571.2, NM_031862.4, NM_001291572.2, NM_005899.5
Summary	The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled-coil motif, which is present in many genes with transformation potential. It functions as a specific autophagy receptor for the selective autophagic degradation of peroxisomes by forming intracellular inclusions with ubiquitylated autophagic substrates. This gene is located on a region of chromosome 17q21.1 that is in close proximity to the BRCA1 tumor suppressor gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.