NRG2 Human shRNA Plasmid Kit (Locus ID 9542)

Specifications

Product Data
Product Name	NRG2 Human shRNA Plasmid Kit (Locus ID 9542)
Locus ID	9542
UniProt ID	O14511
Synonyms	DON1; HRG2; NTAK
Vector	pRS
Format	Retroviral plasmids
Kit Components	NRG2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 9542). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001184935, NM_004883, NM_013981, NM_013982, NM_013983, NM_013984, NM_013985, NM_013981.1, NM_013981.2, NM_013981.3, NM_013982.1, NM_013982.2, NM_004883.1, NM_004883.2, NM_013983.1, NM_013983.2, NM_001184935.1, BC103983, BC103984, BC114333, BC114334, BC166615, NM_004883.3, NM_013982.3, NM_013983.3, NM_013981.4
Summary	This gene encodes a novel member of the neuregulin family of growth and differentiation factors. Through interaction with the ERBB family of receptors, this protein induces the growth and differentiation of epithelial, neuronal, glial, and other types of cells. The gene consists of 12 exons and the genomic structure is similar to that of neuregulin 1, another member of the neuregulin family of ligands. The products of these genes mediate distinct biological processes by acting at different sites in tissues and eliciting different biological responses in cells. This gene is located close to the region for demyelinating Charcot-Marie-Tooth disease locus, but is not responsible for this disease. Alternative transcript variants encoding distinct isoforms have been described. [provided by RefSeq, May 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.