PAX8 Human shRNA Plasmid Kit (Locus ID 7849)

Specifications

Product Data
Product Name	PAX8 Human shRNA Plasmid Kit (Locus ID 7849)
Locus ID	7849
UniProt ID	Q06710
Vector	pRS
Format	Retroviral plasmids
Kit Components	PAX8 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 7849). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_003466, NM_013951, NM_013952, NM_013953, NM_013992, NM_003466.1, NM_003466.2, NM_003466.3, NM_013952.1, NM_013952.2, NM_013952.3, NM_013953.1, NM_013953.2, NM_013953.3, NM_013992.1, NM_013992.2, NM_013992.3, NM_013951.3, BC001060, BC001060.2, BM726535, NM_013992.4, NM_013952.4, NM_013953.4, NM_003466.4
Summary	This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.