PPP1R3C Human shRNA Plasmid Kit (Locus ID 5507)

CAT#: TR310232

PPP1R3C - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided



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CNY 6,790.00


货期*
2周

规格
    • 1 kit

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Specifications

Product Data
Product Name PPP1R3C Human shRNA Plasmid Kit (Locus ID 5507)
Locus ID 5507
UniProt ID Q9UQK1
Synonyms PPP1R5; PTG
Vector pRS
Format Retroviral plasmids
Kit Components PPP1R3C - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 5507). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq NM_005398, NM_005398.1, NM_005398.2, NM_005398.3, NM_005398.4, NM_005398.5, NM_005398.6, BC012625
Summary This gene encodes a carbohydrate binding protein that is a subunit of the protein phosphatase 1 (PP1) complex. PP1 catalyzes reversible protein phosphorylation, which is important in a wide range of cellular activities. The encoded protein affects glycogen biosynthesis by activating glycogen synthase and limiting glycogen breakdown by reducing glycogen phosphorylase activity. DNA hypermethylation of this gene has been found in colorectal cancer patients. The encoded protein also interacts with the laforin protein, which is a protein tyrosine phosphatase implicated in Lafora disease. [provided by RefSeq, Sep 2016]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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