GLEPP1 (PTPRO) Human shRNA Plasmid Kit (Locus ID 5800)

Specifications

Product Data
Product Name	GLEPP1 (PTPRO) Human shRNA Plasmid Kit (Locus ID 5800)
Locus ID	5800
UniProt ID	Q16827
Synonyms	GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT; PTPU2; R-PTP-O
Vector	pRS
Format	Retroviral plasmids
Kit Components	PTPRO - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 5800). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_002848, NM_030667, NM_030668, NM_030669, NM_030670, NM_030671, NM_030668.1, NM_030668.2, NM_030669.1, NM_030669.2, NM_030667.1, NM_030667.2, NM_030670.1, NM_030670.2, NM_002848.1, NM_002848.2, NM_002848.3, NM_030671.1, NM_030671.2, BC126201, BC035960, BC126203, NM_002848.4, NM_030668.3, NM_030670.3, NM_030667.3, NM_030669.3, NM_030671.3
Summary	This gene encodes a member of the R3 subtype family of receptor-type protein tyrosine phosphatases. These proteins are localized to the apical surface of polarized cells and may have tissue-specific functions through activation of Src family kinases. This gene contains two distinct promoters, and alternatively spliced transcript variants encoding multiple isoforms have been observed. The encoded proteins may have multiple isoform-specific and tissue-specific functions, including the regulation of osteoclast production and activity, inhibition of cell proliferation and facilitation of apoptosis. This gene is a candidate tumor suppressor, and decreased expression of this gene has been observed in several types of cancer. [provided by RefSeq, May 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.