Sterol carrier protein 2 (SCP2) Human shRNA Plasmid Kit (Locus ID 6342)

Specifications

Product Data
Product Name	Sterol carrier protein 2 (SCP2) Human shRNA Plasmid Kit (Locus ID 6342)
Locus ID	6342
UniProt ID	P22307
Synonyms	NLTP; NSL-TP; SCOX; SCP-2; SCP-CHI; SCP-X; SCPX
Vector	pRS
Format	Retroviral plasmids
Kit Components	SCP2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 6342). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001007098, NM_001007099, NM_001007100, NM_001007250, NM_001193599, NM_001193600, NM_001193617, NM_002979, NM_001330587, NM_001007098.1, NM_001007098.2, NM_001007099.1, NM_001007099.2, NM_001007250.1, NM_001007250.2, NM_001007100.1, NM_001007100.2, NM_002979.1, NM_002979.2, NM_002979.3, NM_002979.4, NM_001193617.1, NM_001193600.1, NM_001193599.1, BC067108, BC067108.1, BC005911, NM_001193617.2, NM_001193599.2, NM_001193600.2, NM_001007099.3, NM_001007100.3, NM_002979.5
Summary	This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms.[provided by RefSeq, Aug 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.