UBE2V1 (TMEM189-UBE2V1) Human shRNA Plasmid Kit (Locus ID 387522)

Specifications

Product Data
Product Name	UBE2V1 (TMEM189-UBE2V1) Human shRNA Plasmid Kit (Locus ID 387522)
Locus ID	387522
UniProt ID	Q13404
Synonyms	CROC-1B; Kua; Kua-UEV; OTTHUMP00000031794; transmembrane protein 189; UBE2V1; ubiquitin-conjugating enzyme E2 variant 1; ubiquitin-conjugating enzyme variant Kua
Vector	pRS
Format	Retroviral plasmids
Kit Components	TMEM189-UBE2V1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 387522). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_003349, NM_199203, NM_199203.1, NM_199203.2, NM_003349.4, BC172339, BM556348
Summary	The TMEM189-UEV mRNA is an infrequent but naturally occurring read-through transcript of the neighboring TMEM189 and UBE2V1 genes. Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein produced by this transcript has UEV1 B domains but the protein is localized to the cytoplasm rather than to the nucleus. The significance of this read-through mRNA and the function of its protein product has not yet been determined. [provided by RefSeq, Oct 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.