C13orf18 (RUBCNL) Human shRNA Plasmid Kit (Locus ID 80183)

CAT#: TR306223

RUBCNL - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided



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CNY 6,175.00


货期*
3周

规格
    • 1 kit

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RUBCNL Antibody - middle region
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Specifications

Product Data
Product Name C13orf18 (RUBCNL) Human shRNA Plasmid Kit (Locus ID 80183)
Locus ID 80183
UniProt ID Q9H714
Synonyms C13orf18; KIAA0226L; PACER
Vector pRS
Format Retroviral plasmids
Kit Components RUBCNL - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 80183). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq BC032311, BC043488, NM_001286761, NM_001286762, NM_001286763, NM_001286764, NM_001286765, NM_001286766, NM_025113, NM_001349772, NM_025113.1, NM_025113.2, NM_025113.3, NM_025113.4, NM_001286766.1, NM_001286766.2, NM_001286765.1, NM_001286765.2, NM_001286764.1, NM_001286764.2, NM_001286763.1, NM_001286763.2, NM_001286762.1, NM_001286762.2, NM_001286761.1, NM_001286761.2, BC043488.1, BC004495, BC021097, BM682058, NM_001286764.3, NM_001286763.3, NM_025113.5, NM_001286762.3
Summary This gene encodes a cysteine-rich protein that contains a putative zinc-RING and/or ribbon domain. The encoded protein is related to Run domain Beclin-1-interacting and cysteine-rich domain-containing protein, which plays a role in endocytic trafficking and autophagy. In cervical cancer cell lines, this gene is expressed at low levels and may function as a tumor suppressor. Promoter hypermethylation of this gene is observed in cervical cancer cell lines and tissue derived from human patients. [provided by RefSeq, Mar 2017]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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