Caspase-7 (CASP7) Human shRNA Plasmid Kit (Locus ID 840)

Specifications

Product Data
Product Name	Caspase-7 (CASP7) Human shRNA Plasmid Kit (Locus ID 840)
Locus ID	840
UniProt ID	P55210
Synonyms	CASP-7; CMH-1; ICE-LAP3; LICE2; MCH3
Vector	pRS
Format	Retroviral plasmids
Kit Components	CASP7 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 840). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001227, NM_001267056, NM_001267057, NM_001267058, NM_033338, NM_033339, NM_033340, NM_001320911, NM_033339.1, NM_033339.2, NM_033339.3, NM_033339.4, NM_033340.1, NM_033340.3, NM_001227.1, NM_001227.2, NM_001227.3, NM_001227.4, NM_033338.1, NM_033338.2, NM_033338.4, NM_033338.5, NM_001267058.1, NM_001267056.1, NM_001267057.1, BC015799, BC015799.1, BM976488, NM_033340.4, NM_033338.6, NM_001227.5, NM_033339.5
Summary	This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.