Cadherin like 23 (CDH23) Human shRNA Plasmid Kit (Locus ID 64072)

Specifications

Product Data
Product Name	Cadherin like 23 (CDH23) Human shRNA Plasmid Kit (Locus ID 64072)
Locus ID	64072
UniProt ID	Q9H251
Synonyms	CDHR23; PITA5; USH1D
Vector	pRS
Format	Retroviral plasmids
Kit Components	CDH23 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 64072). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	BC032581, NM_001171930, NM_001171931, NM_001171932, NM_001171933, NM_001171934, NM_001171935, NM_001171936, NM_022124, NM_052836, NM_052836.1, NM_052836.2, NM_022124.1, NM_022124.2, NM_022124.3, NM_022124.4, NM_001171936.1, NM_001171935.1, NM_001171932.1, NM_001171931.1, NM_001171934.1, NM_001171933.1, NM_001171930.1, BC011570, BC065284, BC108254, BC136976, BC136977, BC139903, BM673126, NM_001171930.2, NM_001171931.2, NM_052836.4
Summary	This gene is a member of the cadherin superfamily, whose genes encode calcium dependent cell-cell adhesion glycoproteins. The encoded protein is thought to be involved in stereocilia organization and hair bundle formation. The gene is located in a region containing the human deafness loci DFNB12 and USH1D. Usher syndrome 1D and nonsyndromic autosomal recessive deafness DFNB12 are caused by allelic mutations of this cadherin-like gene. Upregulation of this gene may also be associated with breast cancer. Alternative splice variants encoding different isoforms have been described. [provided by RefSeq, May 2013]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.