CSHL1 Human shRNA Plasmid Kit (Locus ID 1444)

Specifications

Product Data
Product Name	CSHL1 Human shRNA Plasmid Kit (Locus ID 1444)
Locus ID	1444
UniProt ID	Q14406
Synonyms	CS-5; CSHP1; CSL; GHB4; hCS-L
Vector	pRS
Format	Retroviral plasmids
Kit Components	CSHL1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 1444). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001318, NM_022578, NM_022579, NM_022580, NM_022581, NM_001321067, NM_001321068, NM_001321069, NM_001318.1, NM_001318.3, NM_022579.1, NM_022579.2, NM_022580.1, NM_022580.2, NM_022581.1, NM_022581.2, BC029365, BC029365.1, BC119747, NM_022581.3, NM_022579.3, NM_001318.4, NM_022580.3
Summary	The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. This particular family member is expressed in placental villi, although it was originally thought to be a pseudogene. In fact, alternative splicing suggests that the majority of the transcripts would be unable to express a secreted protein. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.