Cytoglobin (CYGB) Human shRNA Plasmid Kit (Locus ID 114757)
CAT#: TR305139
CYGB - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
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CNY 4,790.00
货期*
现货
规格
Cited in 1 publication. |
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Specifications
Product Data | |
Product Name | Cytoglobin (CYGB) Human shRNA Plasmid Kit (Locus ID 114757) |
Locus ID | 114757 |
UniProt ID | Q8WWM9 |
Synonyms | HGB; STAP |
Vector | pRS |
Format | Retroviral plasmids |
Kit Components | CYGB - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 114757). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free. |
RefSeq | NM_134268, NM_134268.1, NM_134268.2, NM_134268.3, NM_134268.4, BC029798, BC029798.1, NM_134268.5 |
Summary | This gene encodes a globin protein found in vertebrate cells. The encoded protein is described as a hexacoordinate hemoglobin which binds ligand differently from the pentacoordinate hemoglobins involved in oxygen transport, and may be involved in protection during oxidative stress. This gene is located on chromosome 17 in the same region as a retinal gene which is mutated in progressive rod-cone degeneration, but in the opposite orientation. [provided by RefSeq, Jan 2012] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (1)
The use of this RNAi has been cited in the following citations: |
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Cytoglobin, the Newest Member of the Globin Family, Functions as a Tumor Suppressor Gene
,Narayan Shivapurkar, Victor Stastny, Naoki Okumura, Luc Girard, Yang Xie, Clemens Prinsen, Frederik B. Thunnissen, Ignacio I. Wistuba, Bogdan Czerniak, Eugene Frenkel, Jack A. Roth, Triantafillos Liloglou, George Xinarianos, John K. Field, John D. Minna and Adi F. Gazdar,
Cancer Research 68, 7448, September 15, 2008
[CYGB]
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