EIF4G1 Human shRNA Plasmid Kit (Locus ID 1981)

Specifications

Product Data
Product Name	EIF4G1 Human shRNA Plasmid Kit (Locus ID 1981)
Locus ID	1981
UniProt ID	Q04637
Synonyms	EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18
Vector	pRS
Format	Retroviral plasmids
Kit Components	EIF4G1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 1981). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001194946, NM_001194947, NM_001291157, NM_004953, NM_182917, NM_198241, NM_198242, NM_198244, NM_004953.1, NM_004953.2, NM_004953.3, NM_004953.4, NM_198241.1, NM_198241.2, NM_198242.1, NM_198242.2, NM_198244.1, NM_198244.2, NM_182917.1, NM_182917.2, NM_182917.3, NM_182917.4, NM_001194946.1, NM_001194947.1, NM_001291157.1, BC140892, BC007788, BC010688, BC065256, BC082764, BC140896, BM475154, NM_198242.3, NM_004953.5
Summary	The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.