EYA4 Human shRNA Plasmid Kit (Locus ID 2070)

Specifications

Product Data
Product Name	EYA4 Human shRNA Plasmid Kit (Locus ID 2070)
Locus ID	2070
UniProt ID	O95677
Synonyms	CMD1J; DFNA10
Vector	pRS
Format	Retroviral plasmids
Kit Components	EYA4 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 2070). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001301012, NM_001301013, NM_004100, NM_172103, NM_172104, NM_172105, NM_172103.1, NM_172103.2, NM_172103.3, NM_004100.1, NM_004100.2, NM_004100.3, NM_004100.4, NM_172105.1, NM_172105.2, NM_172105.3, NM_001301012.1, NM_001301013.1, BC041063, BC014193, BC030588, NM_001370458, NM_001370459, NM_004100.5
Summary	This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti. Mutations in this gene are associated with postlingual, progressive, autosomal dominant hearing loss at the deafness, autosomal dominant non-syndromic sensorineural 10 locus. The encoded protein is also a putative oncogene that mediates DNA repair, apoptosis, and innate immunity following DNA damage, cellular damage, and viral attack. Defects in this gene are also associated with dilated cardiomyopathy 1J. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.