FAM111A Human shRNA Plasmid Kit (Locus ID 63901)

Specifications

Product Data
Product Name	FAM111A Human shRNA Plasmid Kit (Locus ID 63901)
Locus ID	63901
UniProt ID	Q96PZ2
Synonyms	GCLEB; KCS2
Vector	pRS
Format	Retroviral plasmids
Kit Components	FAM111A - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 63901). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001142519, NM_001142520, NM_001142521, NM_001312909, NM_001312910, NM_001312911, NM_022074, NM_198847, NM_022074.1, NM_022074.2, NM_022074.3, NM_198847.1, NM_198847.2, NM_001142519.1, NM_001142519.2, NM_001142520.1, NM_001142520.2, NM_001142521.1, NM_001142521.2, BC071759, BC071759.1, BC013137, BC041693, BC054515, NM_001369457, NM_001369455, NM_001369456, NM_001142519.3, NM_001142520.3, NM_001142521.3, NM_198847.3, NM_022074.4
Summary	The protein encoded by this gene is cell-cycle regulated, and has nuclear localization. The C-terminal half of the protein shares homology with trypsin-like peptidases and it contains a PCNA-interacting peptide (PIP) box, that is necessary for its co-localization with proliferating cell nuclear antigen (PCNA). Reduced expression of this gene resulted in DNA replication defects, consistent with the demonstrated role for this gene in Simian Virus 40 (SV40) viral replication. Mutations in this gene have been associated with Kenny-Caffey syndrome (KCS) type 2 and the more severe osteocraniostenosis (OCS, also known as Gracile Bone Dysplasia), both characterized by short stature, hypoparathyroidism, bone development abnormalities, and hypocalcemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.