PPHLN1 Human shRNA Plasmid Kit (Locus ID 51535)

Specifications

Product Data
Product Name	PPHLN1 Human shRNA Plasmid Kit (Locus ID 51535)
Locus ID	51535
UniProt ID	Q8NEY8
Synonyms	CR; HSPC206; HSPC232
Vector	pRS
Format	Retroviral plasmids
Kit Components	PPHLN1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 51535). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001143787, NM_001143788, NM_001143789, NM_016488, NM_201438, NM_201439, NM_201440, NM_201515, NM_201438.1, NM_016488.1, NM_016488.2, NM_016488.3, NM_016488.4, NM_016488.5, NM_016488.6, NM_201515.1, NM_201439.1, NM_201440.1, NM_001143787.1, NM_001143788.1, NM_001143789.1, BC039832, BC039832.1, BC015322, BC025306, BC040449, BM995486, NM_001364829, NM_001364823, NM_001364824, NM_001364825, NM_001364827, NM_001364828, NM_001364830, NM_001364831, NM_001364833, NM_001364822, NM_001364826, NM_001364832, NM_001364834, NM_001143787.2, NM_201438.2, NM_201439.2, NM_016488.7
Summary	The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.