RNF14 Human shRNA Plasmid Kit (Locus ID 9604)

Specifications

Product Data
Product Name	RNF14 Human shRNA Plasmid Kit (Locus ID 9604)
Locus ID	9604
UniProt ID	Q9UBS8
Synonyms	ARA54; HFB30; HRIHFB2038; TRIAD2
Vector	pRS
Format	Retroviral plasmids
Kit Components	RNF14 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 9604). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001201365, NM_004290, NM_183398, NM_183399, NM_183400, NM_183401, NM_004290.1, NM_004290.2, NM_004290.4, NM_183400.1, NM_183400.2, NM_183401.1, NM_183401.2, NM_183398.1, NM_183398.2, NM_183399.1, NM_183399.2, NM_001201365.1, BC126185, BC144061, NM_183400.3, NM_183401.3, NM_004290.5, NM_183399.3, NM_001201365.2
Summary	The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.