Nlrp3 Mouse shRNA Plasmid (Locus ID 216799)
CAT#: TG510752
Nlrp3 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
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CNY 4,790.00
货期*
现货
规格
Cited in 8 publications. |
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经常一起买 (2)
Specifications
Product Data | |
Product Name | Nlrp3 Mouse shRNA Plasmid (Locus ID 216799) |
Locus ID | 216799 |
UniProt ID | Q8R4B8 |
Synonyms | AGTAVPRL; AII/AVP; Cias1; FCAS; FCU; Mmig1; MWS; NALP3; Pypaf1 |
Vector | pGFP-V-RS |
Format | Retroviral plasmids |
Kit Components | Nlrp3 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 216799). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free. |
RefSeq | BC116174, BC116175, NM_145827, NM_001359638, NR_153314, NM_145827.1, NM_145827.2, NM_145827.3 |
Summary | As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (or possibly CASP4/CASP11). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu (PubMed:28847925). Activation of NLRP3 inflammasome is also required for HMGB1 secretion (PubMed:22801494). The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K(+) efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33 (By similarity). Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth. During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (PubMed:26098997).[UniProtKB/Swiss-Prot Function] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (8)
The use of this RNAi has been cited in the following citations: |
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Contribution of cathepsin B-dependent Nlrp3 inflammasome activation to nicotine-induced endothelial barrier dysfunction
,Zhang, Y;Chen, Y;Zhang, Y;Li, PL;Li, X;,
Eur. J. Pharmacol.
,PubMed ID 31733211
[NLRP3]
|
Procyanidin B2 protects rats from paraquat-induced acute lung injury by inhibiting NLRP3 inflammasome activation.
,null,
Immunobiology
,PubMed ID 30025709
[Nlrp3]
|
Enhancement of endothelial permeability by free fatty acid through lysosomal cathepsin B-mediated Nlrp3 inflammasome activation
,Wang, L;Chen, Y;Li, X;Zhang, Y;Gulbins, E;Zhang, Y;,
Oncotarget
,PubMed ID 27689324
[NLRP3]
|
Contribution of redox-dependent activation of endothelial Nlrp3 inflammasomes to hyperglycemia-induced endothelial dysfunction
,Chen, Y;Wang, L;Pitzer, AL;Li, X;Li, PL;Zhang, Y;,
J. Mol. Med.
,PubMed ID 27783111
[Nlrp3]
|
Involvement of the P2X7-NLRP3 axis in leukemic cell proliferation and death
,null,
Scientific Reports
,PubMed ID 27221966
[Nlrp3]
|
Inhibition of Hyperhomocysteinemia-Induced Inflammasome Activation and Glomerular Sclerosis by NLRP3 Gene Deletion
,null,
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
,PubMed ID 25171193
[Nlrp3]
|
Instigation of endothelial Nlrp3 inflammasome by adipokine visfatin promotes inter-endothelial junction disruption: role of HMGB1
,Chen, Y;Pitzer, AL;Li, X;Li, PL;Wang, L;Zhang, Y;,
J. Cell. Mol. Med.
,PubMed ID 26293846
[NLRP3]
|
Endothelial Nlrp3 Inflammasome Activation Associated with Lysosomal Destabilization during Coronary Arteritis
,null,
Biochimica et biophysica acta
,PubMed ID 25450976
[Nlrp3]
|
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