ENT1 (SLC29A1) Human shRNA Plasmid Kit (Locus ID 2030)
CAT#: TG309346
SLC29A1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
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CNY 4,790.00
货期*
现货
规格
Cited in 1 publication. |
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经常一起买 (2)
Specifications
Product Data | |
Product Name | ENT1 (SLC29A1) Human shRNA Plasmid Kit (Locus ID 2030) |
Locus ID | 2030 |
UniProt ID | Q99808 |
Synonyms | ENT1 |
Vector | pGFP-V-RS |
Format | Retroviral plasmids |
Kit Components | SLC29A1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 2030). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free. |
RefSeq | NM_001078174, NM_001078175, NM_001078176, NM_001078177, NM_001304462, NM_001304463, NM_001304465, NM_001304466, NM_004955, NM_004955.1, NM_004955.2, NM_001078175.1, NM_001078175.2, NM_001078174.1, NM_001078176.1, NM_001078176.2, BC001382, BC001382.2, BC008954, BC008954.2, BC095398, BM661584, NM_001078177.2, NM_001078175.3 |
Summary | This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (1)
The use of this RNAi has been cited in the following citations: |
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SLC29A1 (ENT1) polymorphisms and outcome of complete remission in acute myeloid leukemia
,Kim, JH;Lee, C;Cheong, HS;Koh, Y;Ahn, KS;Kim, HL;Shin, HD;Yoon, SS;,
Cancer Chemother. Pharmacol.
,PubMed ID 27422302
[SLC29A1]
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