ARID1A Human shRNA Plasmid Kit (Locus ID 8289)
CAT#: TG306603
ARID1A - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
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CNY 4,790.00
货期*
现货
规格
Cited in 2 publications. |
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经常一起买 (2)
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Specifications
Product Data | |
Product Name | ARID1A Human shRNA Plasmid Kit (Locus ID 8289) |
Locus ID | 8289 |
UniProt ID | O14497 |
Synonyms | B120; BAF250; BAF250a; BM029; C1orf4; CSS2; ELD; hELD; hOSA1; MRD14; OSA1; P270; SMARCF1 |
Vector | pGFP-V-RS |
Format | Retroviral plasmids |
Kit Components | ARID1A - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 8289). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free. |
RefSeq | NM_006015, NM_018450, NM_139135, NM_006015.1, NM_006015.2, NM_006015.3, NM_006015.4, NM_139135.1, NM_139135.2, BC112895, NM_139135.4, NM_006015.6 |
Summary | This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (2)
The use of this RNAi has been cited in the following citations: |
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A signaling cascade including ARID1A, GADD45B and DUSP1 induces apoptosis and affects the cell cycle of germ cell cancers after romidepsin treatment
,Nettersheim, D;Jostes, S;Fabry, M;Honecker, F;Schumacher, V;Kirfel, J;Kristiansen, G;Schorle, H;,
Oncotarget
,PubMed ID 27572311
[ARID1A]
|
BAF complexes facilitate decatenation of DNA by topoisomerase IIa
,Emily C. Dykhuizen, Diana C. Hargreaves, Erik L. Miller, Kairong Cui, Andrey Korshunov, + et al.,
Nature 497, 624-627 doi:10.1038/nature12146
,PubMed ID 23698369
[Arid1a]
|
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