GPR92 (LPAR5) Human shRNA Plasmid Kit (Locus ID 57121)
CAT#: TG304222
LPAR5 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
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CNY 4,790.00
货期*
现货
规格
Cited in 7 publications. |
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经常一起买 (2)
Specifications
Product Data | |
Product Name | GPR92 (LPAR5) Human shRNA Plasmid Kit (Locus ID 57121) |
Locus ID | 57121 |
UniProt ID | Q9H1C0 |
Synonyms | GPR92; GPR93; KPG_010; LPA5 |
Vector | pGFP-V-RS |
Format | Retroviral plasmids |
Kit Components | LPAR5 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 57121). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free. |
RefSeq | NM_001142961, NM_020400, NM_020400.1, NM_020400.2, NM_020400.3, NM_020400.4, NM_020400.5, NM_001142961.1, BC072394, BC072394.1, BC033571, NM_020400.6 |
Summary | This gene encodes a member of the rhodopsin class of G protein-coupled transmembrane receptors. This protein transmits extracellular signals from lysophosphatidic acid to cells through heterotrimeric G proteins and mediates numerous cellular processes. Many G protein receptors serve as targets for pharmaceutical drugs. Transcript variants of this gene have been described.[provided by RefSeq, Dec 2008] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (7)
The use of this RNAi has been cited in the following citations: |
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Different effects of lysophosphatidic acid receptor-2 (LPA2) and LPA5 on the regulation of chemoresistance in colon cancer cells
,Ishimoto, K;Minami, A;Minami, K;Ueda, N;Tsujiuchi, T;,
J. Recept. Signal Transduct. Res.
,PubMed ID 32672083
[LPAR5]
|
LPA5-mediated signaling induced by endothelial cells and anticancer drug regulates cellular functions of osteosarcoma cells
,Minami, K;Ueda, N;Ishimoto, K;Tsujiuchi, T;,
Experimental Cell Research
,PubMed ID 31904382
[LPAR5]
|
Modulation of chemoresistance by lysophosphatidic acid (LPA) signaling through LPA5 in melanoma cells treated with anticancer drugs
,Minami, K;Ueda, N;Maeda, H;Ishimoto, K;Otagaki, S;Tsujiuchi, T;,
Biochem. Biophys. Res. Commun.
,PubMed ID 31362892
[LPAR5]
|
Diverse effects of LPA4, LPA5 and LPA6 on the activation of tumor progression in pancreatic cancer cells
,Ishii, S;Hirane, M;Fukushima, K;Tomimatsu, A;Fukushima, N;Tsujiuchi, T;,
Biochem. Biophys. Res. Commun.
,PubMed ID 25849892
[LPAR5]
|
Inhibitory effects of lysophosphatidic acid receptor-5 on cellular functions of sarcoma cells
,Araki, M;Kitayoshi, M;Dong, Y;Hirane, M;Ozaki, S;Mori, S;Fukushima, N;Honoki, K;Tsujiuchi, T;,
Growth Factors
,PubMed ID 24798396
[LPAR5]
|
Lysophosphatidic acid receptor-5 negatively regulates cell motile and invasive activities of human sarcoma cell lines
,Dong, Y;Hirane, M;Araki, M;Fukushima, N;Honoki, K;Tsujiuchi, T;,
Mol. Cell. Biochem. March 2014
,PubMed ID 24676544
[LPAR5]
|
Lysophosphatidic acid receptor-5 negatively regulates cellular responses in mouse fibroblast 3T3 cells
,Dong, Y;Hirane, M;Araki, M;Fukushima, N;Tsujiuchi, T;,
Biochem. Biophys. Res. Commun.
,PubMed ID 24632199
[Lpar5]
|
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