TFII I (GTF2I) Human shRNA Plasmid Kit (Locus ID 2969)

Specifications

Product Data
Product Name	TFII I (GTF2I) Human shRNA Plasmid Kit (Locus ID 2969)
Locus ID	2969
UniProt ID	P78347
Synonyms	BAP135; BTKAP1; DIWS; GTFII-I; IB291; SPIN; TFII-I; WBS; WBSCR6
Vector	pGFP-V-RS
Format	Retroviral plasmids
Kit Components	GTF2I - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 2969). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq	NM_001163636, NM_001280800, NM_001518, NM_032999, NM_033000, NM_033001, NM_033003, NM_001518.1, NM_001518.2, NM_001518.3, NM_001518.4, NM_033001.1, NM_033001.2, NM_033001.3, NM_032999.1, NM_032999.2, NM_032999.3, NM_033000.1, NM_033000.2, NM_033000.3, NM_001163636.1, NM_001163636.2, NM_001280800.1, BC070484, BC070484.1, BC099907, BC099907.1, BC004472, NM_001163636.3
Summary	This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.